GST-tagged fusion protein - rapid purification, detection - Database & Sql Blog Articles

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GST Tag Fusion Protein - Purification and Detection

Glutathione S-transferase (GST) is a protein composed of 211 amino acids that is commonly added to the end of recombinant proteins for purification or detection purposes. Unlike histidine tags, which can cause non-specific binding and hinder affinity purification, the GST tag offers high specificity due to its strong interaction with glutathione. This leads to higher purity in the final product.

GST Tag Protein Purification Tool

Use GSH agarose gel or magnetic beads for efficient purification of GST fusion proteins and GSH-binding proteins. The PurKineâ„¢ GST-Tag Purification System utilizes an advanced high-load matrix that enables single-step purification of recombinant GST fusion proteins from both E. coli and mammalian cell lysates. This system is optimized to ensure the stability, solubility, and yield of purified proteins. Pre-packed columns and kits are also available for added convenience. In addition, Abbkine provides agarose resin and magnetic bead options for flexible use.

The PurKineâ„¢ GST-Tag Glutathione Resin 4FF demonstrates superior elution efficiency and protein loading capacity compared to similar products from other brands.

Fig. Comparison of GST recombinant protein purification results: PurKineâ„¢ GST-Tag Glutathione Resin 4FF (Lane B), Supplier G products (Lane C).

Precautions:

The GST tag has mild elution conditions, which helps preserve protein activity and is suitable for pull-down experiments. It also offers a good linear dynamic range. However, it has a relatively large molecular weight, which may affect the function of the target protein and interfere with downstream experiments. If the protein is insoluble, purification through denaturation may be challenging.

GST Tag Protein Detection Antibody

Detect GST-tagged fusion proteins using GST-specific antibodies. These antibodies are widely used in Western blot (WB) experiments to evaluate the effectiveness of GST-tag purification and determine if the recombinant protein has been degraded.

As the official agent of Abbkine China, Amy Jiejie offers cost-effective immunological reagents such as various tag antibodies, internal reference antibodies, conventional primary antibodies, secondary antibodies, detection substrates, protein purification products, and related kits. As an authorized agent for several international brands, Aimei can provide comprehensive laboratory solutions. If you are interested in these products, feel free to contact Ai Meijie Technology Co., Ltd. for detailed information and complete experimental support.