FOR OPEL Brake Disc Opel Brake Disc,Opel Auto Brake Disc,Opel Car Brake Disc,Opel Automobile Brake Disc Zhoushan Shenying Filter Manufacture Co., Ltd. , https://www.renkenfilter.com
FOR OPEL Brake Disc Opel Brake Disc,Opel Auto Brake Disc,Opel Car Brake Disc,Opel Automobile Brake Disc Zhoushan Shenying Filter Manufacture Co., Ltd. , https://www.renkenfilter.com
On the day before, Mr. Xia from Southwest University contacted our company via phone and purchased an ELISA kit. During the call, Mr. Xia and her manager, Mr. He, had a live Q&A session to clarify the sample preparation procedures.
Mr. Xia asked: "How should serum samples be handled?"
Mr. He replied: "For whole blood samples, allow them to sit at room temperature for two hours or leave them overnight at 4°C. Then centrifuge at 1000 × g for 20 minutes. Carefully collect the supernatant. If precipitation occurs during storage, repeat the centrifugation."
Mr. Xia then asked: "What about plasma samples?"
Mr. He explained: "Choose an anticoagulant such as EDTA, sodium citrate, or heparin based on your requirements. Mix the sample for 10–20 minutes, then centrifuge at 1000 × g for 15 minutes. Collect the supernatant carefully. Again, if precipitation occurs, you can centrifuge again."
Next, Mr. Xia inquired: "How do we prepare cell supernatant samples?"
Mr. He responded: "When detecting secreted components, collect the supernatant using sterile tubes and centrifuge for approximately 20 minutes at 1000 × g. For intracellular component detection, dilute the cell suspension with PBS (pH 7.2–7.4) to reach a concentration of around 1 million cells per mL. Use repeated freeze-thaw cycles to lyse the cells, then centrifuge for about 10 minutes at 5000 × g. Carefully collect the supernatant. If any precipitate forms, re-centrifuge."
Finally, Mr. Xia asked: "Why do some kits use competitive ELISA?"
Mr. He explained: "Competitive ELISA is used when dealing with small molecule antigens or haptens that cannot be measured by the sandwich method due to lacking multiple binding sites. In this method, the antigen in the sample competes with the antigen coated on the solid phase for binding to the biotinylated antibody. The more antigen present in the sample, the less biotinylated antibody binds to the plate, resulting in a lighter color development. This means the color intensity is inversely proportional to the antigen concentration. This technique is commonly used for small molecules like hormones and drugs."
We hope the above information was helpful. If you have any further questions or need additional support, feel free to reach out to us anytime. We’re always here to assist!
EL-C1600N100013-B KEMET talk about capacitor conventional T491D157M010AT original spot Test - lowercase jpg Kaixin micro test Test probe P100-M3