OTHERS FOR Fuel Filter Others Fuel Filter,Others Auto Fuel Filter,Others Automobile Fuel Filter,Others Car Fuel Filter Zhoushan Shenying Filter Manufacture Co., Ltd. , https://www.renkenfilter.com
OTHERS FOR Fuel Filter Others Fuel Filter,Others Auto Fuel Filter,Others Automobile Fuel Filter,Others Car Fuel Filter Zhoushan Shenying Filter Manufacture Co., Ltd. , https://www.renkenfilter.com
Last day, Mr. Xia from Southwest University contacted us via phone and purchased our ELISA kit after a detailed discussion. During the call, Mr. Xia and his manager, Mr. He, had an online Q&A session that covered several important technical points.
Mr. Xia asked: "How should serum samples be handled?"
Manager replied: "For whole blood samples, allow them to sit at room temperature for 2 hours or overnight at 4°C before centrifuging at 1000 × g for 20 minutes. Carefully collect the supernatant. If a precipitate forms during storage, re-centrifuge."
Mr. Xia then asked: "What about plasma samples?"
Manager responded: "Choose an anticoagulant such as EDTA, sodium citrate, or heparin depending on the sample requirements. Mix for 10–20 minutes, then centrifuge at 1000 × g for 15 minutes. Collect the supernatant carefully. Again, if a precipitate appears, re-centrifuge."
Mr. Xia continued: "How to prepare cell supernatant samples?"
Manager explained: "When detecting secreted components, collect the supernatant using sterile tubes and centrifuge for about 20 minutes at 1000 × g. For intracellular components, dilute the cell suspension with PBS (pH 7.2–7.4) to reach approximately 1 million cells per mL. Use repeated freeze-thaw cycles to lyse the cells, then centrifuge for about 10 minutes at 5000 × g. Carefully collect the supernatant again. If any precipitate is observed, re-centrifuge."
Mr. Xia also asked: "Why do some kits use competitive ELISA?"
Manager answered: "This method is used when dealing with small molecule antigens or haptens, which lack multiple binding sites required for the sandwich method. In competitive ELISA, the antigen in the sample competes with the immobilized antigen for binding to the biotinylated antibody. The more antigen present in the sample, the less biotinylated antibody binds to the solid phase, resulting in a lighter color development. This inverse relationship between color intensity and antigen concentration makes this method ideal for detecting small molecules like hormones and drugs."
We hope this information was helpful. If you have any further questions or need assistance, feel free to reach out to us anytime. We're always here to help!
EL-C1600N100013-B KEMET talk about capacitor conventional T491D157M010AT original spot Test - lowercase jpg Kaixin micro test Test probe P100-M3