Do a good job of prevention, can reduce the occurrence of cell culture pollution - Database & Sql Blog Articles

Are you a cell culture researcher facing the challenge of contamination? It’s a common issue that can ruin your experiments and waste valuable time. The key to success lies in effective prevention. Without proper precautions, even the smallest mistake can lead to serious contamination. So how can you ensure a clean and safe environment for your cell cultures?

Here are some essential tips from Shanghai Hengyuan Bio to help you prevent contamination during your cell culture experiments:

1. Sterilize all equipment and materials thoroughly

All tools, glassware, and reagents should be properly cleaned and sterilized before use. Make sure that any solution you prepare is tested for sterility before it's used. Regularly disinfect the lab space and all sterile equipment to maintain a controlled environment.

2. Focus on operator hygiene and behavior

The person conducting the experiment plays a critical role in preventing contamination. Always wash your hands with soap or a 5% chlorhexidine solution for at least 5 minutes before entering the sterile area. Wear a lab coat as required and avoid unnecessary movement inside the room. Before starting, wipe your hands with 75% alcohol to minimize microbial transfer.

It's also important to work carefully and avoid talking or sneezing over the culture. If you need to cough or sneeze, turn away from the sterile area. Never touch the mouth of pipettes or other tools with your hands, and always replace them after each use to avoid cross-contamination.

3. Prevent cross-contamination between cell lines

Make sure that all instruments are clearly labeled and used only for specific cell types. Avoid using the same syringe or dropper for different cultures. Keep all materials organized and handle them in a logical sequence to reduce the risk of mixing up samples.

4. Maintain cleanliness and order in the lab

After finishing your work, clean the lab bench with disinfectant-soaked gauze. Keep the workspace tidy and avoid leaving any unused items out. A clean and well-organized lab not only improves efficiency but also reduces the chances of contamination.

Shanghai Hengyuan Bio reminds you: Proper contamination control is vital for successful cell culture. Once cells are introduced into the lab, they should be stored properly and monitored regularly. If contamination occurs, it may be difficult or impossible to recover the culture. Prevention is always better than cure.